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SRX10806042: Vision Exome Illumina reads of Batrachostomus septimus run 2
1 ILLUMINA (Illumina MiSeq) run: 289,886 spots, 172.8M bases, 97Mb downloads

Design: Genomic DNA sheared via sonication, Illumina libraries prepared, in-solution hybridization to biotinylated RNA probes for vision exome, sequenced on Illumina platform
Submitted by: National Eye Institute
Study: Extracting phototransduction cascade genes from birds
show Abstracthide Abstract
We developed a probe set that captures exonic regions of 46 genes representing the phototransduction cascade and other light responses. We successfully capture an average of 64 percent of our targeted exome in 46 species across 14 orders. For a subset of species, we directly compare gene capture between our probe set and low-coverage whole genome sequencing. This BioProject contains Illumina reads of both captured vision gene sequence, as well as the shotgun whole genome sequences. Reference: White et al. 2021, A Novel Exome Probe Set Captures Phototransduction Genes Across Birds (Aves), Enabling Efficient Analysis of Vision Evolution. Accepted at Molecular Ecology Resources August 2021.
Sample:
SAMN12662050 • SRS5338662 • All experiments • All runs
Library:
Name: Batrachostomus_septimus_2
Instrument: Illumina MiSeq
Strategy: Targeted-Capture
Source: GENOMIC
Selection: Hybrid Selection
Layout: PAIRED
Runs: 1 run, 289,886 spots, 172.8M bases, 97Mb
Run# of Spots# of BasesSizePublished
SRR14455298289,886172.8M97Mb2021-08-24

ID:
14344909

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